{"id":450,"date":"2016-08-08T11:20:25","date_gmt":"2016-08-08T08:20:25","guid":{"rendered":"http:\/\/infeksiyon.org\/?p=450"},"modified":"2016-08-08T11:20:25","modified_gmt":"2016-08-08T08:20:25","slug":"cytomegalovirus-asisinda-ilk-adim-tamamlandi","status":"publish","type":"post","link":"https:\/\/infeksiyon.org\/en\/cytomegalovirus-asisinda-ilk-adim-tamamlandi\/","title":{"rendered":"A Vaccine Based on the Rhesus Cytomegalovirus UL128 Complex Induces Broadly Neutralizing Antibodies in Rhesus Macaques"},"content":{"rendered":"<p style=\"text-align: justify;\"><span style=\"font-size: medium;\">Neutralizing antibodies (NAb) are important for interfering with horizontal transmission of human cytomegalovirus (HCMV) leading to primary and congenital HCMV infection. Recent findings have shown that a pentameric virion complex formed by the glycoproteins gH\/gL, UL128, UL130, and UL131A (UL128C) is required for HCMV entry into epithelial\/endothelial cells (Epi\/EC) and is the target of potent NAb in HCMV-seropositive individuals. Using bacterial artificial chromosome technology, we have generated a modified vaccinia Ankara virus (MVA) that stably coexpresses all 5 rhesus CMV (RhCMV) proteins homologous to HCMV UL128C, termed MVA-RhUL128C. Coimmunoprecipitation confirmed the interaction of RhgH with the other 4 RhCMV subunits of the pentameric complex. All 8 RhCMV-na\u00efve rhesus macaques (RM) vaccinated with MVA-RhUL128C developed NAb that blocked infection of monkey kidney epithelial cells (MKE) and rhesus fibroblasts. NAb titers induced by MVA-RhUL128C measured on both cell types at 2 to 6 weeks postvaccination were comparable to levels observed in naturally infected RM. In contrast, MVA expressing a subset of RhUL128C proteins or RhgB glycoprotein only minimally stimulated NAb that inhibited infection of MKE. In addition, following subcutaneous RhCMV challenge at 8 weeks postvaccination, animals vaccinated with MVA-RhUL128C showed reduced plasma viral loads. These results indicate that MVA expressing the RhUL128C induces NAb inhibiting RhCMV entry into both Epi\/EC and fibroblasts and limits RhCMV replication in RM. This novel approach is the first step in developing a prophylactic HCMV vaccine designed to interfere with virus entry into major cell types permissive for viral replication, a required property of an effective vaccine.<br \/>\n<b><br \/>\n&#8230;<br \/>\n<\/b><a href=\"http:\/\/jvi.asm.org\/content\/87\/3\/1322.long\" target=\"_blank\">http:\/\/jvi.asm.org\/content\/87\/<wbr \/>3\/1322.long<\/a><b><\/b><\/span><\/p>\n<p>Haber: Hasan Naz<\/p>\n<div style=\"text-align: justify;\"><\/div>\n<h1 style=\"text-align: justify;\"><span style=\"font-size: medium;\">Posted September 23, Eyl\u00fcl 2013<br \/>\n<\/span><\/h1>\n<p>&nbsp;","protected":false},"excerpt":{"rendered":"<p>Neutralizing antibodies (NAb) are important for interfering with horizontal transmission of human cytomegalovirus (HCMV) leading to primary and congenital HCMV infection. Recent findings have shown that a pentameric virion complex formed by the glycoproteins gH\/gL, UL128, UL130, and UL131A (UL128C) is required for HCMV entry into epithelial\/endothelial cells (Epi\/EC) and is the target of potent&#8230; <\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_links_to":"","_links_to_target":""},"categories":[9],"tags":[],"_links":{"self":[{"href":"https:\/\/infeksiyon.org\/en\/wp-json\/wp\/v2\/posts\/450"}],"collection":[{"href":"https:\/\/infeksiyon.org\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/infeksiyon.org\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/infeksiyon.org\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/infeksiyon.org\/en\/wp-json\/wp\/v2\/comments?post=450"}],"version-history":[{"count":1,"href":"https:\/\/infeksiyon.org\/en\/wp-json\/wp\/v2\/posts\/450\/revisions"}],"predecessor-version":[{"id":451,"href":"https:\/\/infeksiyon.org\/en\/wp-json\/wp\/v2\/posts\/450\/revisions\/451"}],"wp:attachment":[{"href":"https:\/\/infeksiyon.org\/en\/wp-json\/wp\/v2\/media?parent=450"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/infeksiyon.org\/en\/wp-json\/wp\/v2\/categories?post=450"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/infeksiyon.org\/en\/wp-json\/wp\/v2\/tags?post=450"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}